Biology experiment dealing with plant organelles and extraction of DNA

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Isolation of Chloroplasts, Mitochondria & Extration of Plant Genomic DNA

Our Price: $95.00

Product Code: 910 EV

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Description

Advanced Experiment for 6 groups

This is a three-part experiment that deals with isolation and extraction of DNA. Two cell organelles are isolated from pea seedlings by differential centrifugation. First, students identify mitochondria by the enzyme activity using the enzyme cytochrome c oxidase. Secondly, organelles and chloroplasts are identified under the microscope. Thirdly, a simple method is provided to extract plant genomic DNA.

Features

Experiment Includes
- Complete instructions, background information and study questions.
- Genomic DNA Extraction Solutions
- Chloroplast Isolation Reagents
- Mitochondrial DNA Isolation Reagents
- Transfer Pipets
- Pea Seeds
- UltraSpec-Agarose
- Concentrated 50x Electrophoresis Buffer
- 10x Gel Loading Solution
- Practice Gel Loading Solution
- InstaStain Methylene Blue
- Methylene Blue Plus
- Glass Rods
- 1 ml Pipets
- Microcentrifuge tubes
- Miracloth
Requirements
- Microscope and slides
- Spectrophotometer
- Blender
- Waterbath (65 degree C)
- Microcentrifuge and Centrifuge Tubes
- Clinical Tabletop Centrifuge (Optional)
- Pipet Pumps or bulbs
- Cheesecloth
- Distilled Water, Ice Bucket and ice
- Acetone (optional)
- Vermiculite
- Nursery Flat 21x10c2 in
- Automatic Micropipet and Tips
- Test Tubes (13 x 100 mm) & Laboratory glassware
- Horizontal Gel Electrophoresis Apparatus
- DC Power Supply
- White Light Visualization System
- Scissors or razor blades
- Pasteur pipets
- funnels
- Small Paint Brushes
- 100% Isopropanol
- 70% and 95% Ethanal
- Mortor and Pestile
Time Requirements - Requires 5.5 hours for completion over three consecutive lab periods

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